Composite

Part:BBa_K331016

Designed by: Lisza Bruder   Group: iGEM10_Lethbridge   (2010-07-01)


IPTG Inducible Catechol 2,3-dioxygenase with N-terminal Arginine Tag

This part is IPTG inducible to produce catechol 1,2-dioxygenase with an N-terminal arginine tag. The arginine sequence allows for the targeting of ECFP into the Lumazine Synthase microcompatment (Bba_K249003).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 573
    Illegal NgoMIV site found at 745
    Illegal AgeI site found at 1096
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None